Difference between revisions of "Part:BBa K2533045"
 
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<h1>'''Usage and biology'''</h1>
 
<h1>'''Usage and biology'''</h1>
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mleS refers to malate dehydrogenase, enhancing the conversion of malic acid to L-lactate. Rhodopseudomonas palustris could utilize lactate more efficiently, which brings Shewanella more carbon sources.
+
mleS refers to malate dehydrogenase, performing the conversion of malic acid to L-lactate. Rhodopseudomonas palustris could produce lactate more efficiently, which brings Shewanella more carbon source.
  
 
<h1>'''Characterization'''</h1>
 
<h1>'''Characterization'''</h1>
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This is one section for lactate utilization part.
+
This is one section for lactate producing part.
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[[File:T--HUST-China--2018-tonglu-mleS.png ‎|400px|thumb|center|Figure1:RBS-mles]]
+
[[File:T--HUST-China--2018-tonglu-mleS.png ‎|400px|thumb|center|Figure1. RBS-mles]]
  
 
<h2>DNA Gel Electrophoretic</h2>
 
<h2>DNA Gel Electrophoretic</h2>
−
To make sure that we get the target gene, we did the DNA gel electrophoretic to separate different gene. And here is the result.
+
To make sure that we get the target gene, we did DNA gel electrophoretic for verification. And here is the result.
−
[[File:T--HUST-China--2018-tonglu-mles.png|400px|thumb|center|Figure2:Verification of successful transformation of pSB1C3-RBS-mleS]]
+
[[File:T--HUST-China--2018-tonglu-mles.png|400px|thumb|center|Figure2. Verification of successful transformation of pSB1C3-RBS-mleS]]
−
Our target genes are 1969bp, and as the marker is DS5000, we could be sure that the bright bands in this picture are our target genes.
+
Our target gene is 1641bp, and as the marker is DS5000, we could be sure that the bright bands in this picture are our target genes.
  
 
<h2>Electrogenesis</h2>
 
<h2>Electrogenesis</h2>
−
By detecting the production of lactate after expressing protein, we might find out whether mleS could effectively help Rhodopseudomonas palustris to produce more lactate.
+
By detecting the production of lactate after expressing, we might find out whether mleS could effectively help Rhodopseudomonas palustris produce more lactate.
−
[[File:T--HUST-China--2018-expression of lactate.png ‎|400px|thumb|center|Figure3:shows that our modification is effective. Every gene circuits can help strains produce lactate, and mleS-lldP-ldhA is the most efficient one. Therefore, our construction of gene circuits achieve the goal to help strains produce lactate.]]
+
[[File:T--HUST-China--2018-expression of lactate.png ‎|400px|thumb|center|Figure3. shows that our modification is effective. Every gene circuits can help strains produce lactate, and mleS-lldP-ldhA is the most efficient one. Therefore, our construction of gene circuits achieves the goal to help strains produce lactate.]]
−
It could be demonstrated that targeted genes could be expressed in the engineered cells. More lactate has been produced by engineered bacteria.  
+
It could be demonstrated that the target genes could be expressed in the engineered cells. More lactate has been produced by engineered bacteria.  
  
 
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Latest revision as of 23:04, 17 October 2018


RBS-mleS

produce lactate

Usage and biology

mleS refers to malate dehydrogenase, performing the conversion of malic acid to L-lactate. Rhodopseudomonas palustris could produce lactate more efficiently, which brings Shewanella more carbon source.

Characterization

This is one section for lactate producing part.

Figure1. RBS-mles

DNA Gel Electrophoretic

To make sure that we get the target gene, we did DNA gel electrophoretic for verification. And here is the result.

Figure2. Verification of successful transformation of pSB1C3-RBS-mleS

Our target gene is 1641bp, and as the marker is DS5000, we could be sure that the bright bands in this picture are our target genes.

Electrogenesis

By detecting the production of lactate after expressing, we might find out whether mleS could effectively help Rhodopseudomonas palustris produce more lactate.

Figure3. shows that our modification is effective. Every gene circuits can help strains produce lactate, and mleS-lldP-ldhA is the most efficient one. Therefore, our construction of gene circuits achieves the goal to help strains produce lactate.

It could be demonstrated that the target genes could be expressed in the engineered cells. More lactate has been produced by engineered bacteria.