Difference between revisions of "Part:BBa K2623008"

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<partinfo>BBa_K2623008 short</partinfo>
 
<partinfo>BBa_K2623008 short</partinfo>
 
===Summary===
 
===Summary===
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This part contains the coding region of the Hypsibius dujardini (Water bear) (Macrobiotus dujardini) CAHS gene. Cytosolic abundant heat soluble proteins acts as a molecular shield in water-deficient condition. Tardigrade-specific intrinsically disordered proteins (TDPs) are essential for desiccation tolerance by forming non-crystalline amorphous solids upon desiccation, and this vitrified state mirrors their protective capabilities.<br>
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This part contains the coding region of the Hypsibius dujardini (Water bear) (Macrobiotus dujardini) CAHS gene. Cytosolic abundant heat soluble proteins acts as a molecular shield in water-deficient condition. Tardigrade-specific intrinsically disordered proteins (TDPs) are essential for desiccation tolerance by forming non-crystalline amorphous solids upon desiccation, and this vitrified state mirrors their protective capabilities.
 
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<table><tr><th>[[Image:CAHS Fig1.png|thumb|300px|]]</th><th></table>
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https://static.igem.org/mediawiki/parts/4/4e/CAHS_Fig1.png
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As you can see, when the water bear is suffering slow drying, the TDPs will upregulate, and then he will become a crystal. Magically, after rehydration, he is still alive. We chose the CAHS1 which play a important role in this process.<br>
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A separate fragment is 714bp.<br>
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<table><tr><th>[[Image:CAHS_protein_Gel.png|thumb|300px|Fig.1 The result of enzyme identification]]</th><th></table>
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https://static.igem.org/mediawiki/parts/4/4f/CAHS_protein_Gel.png
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As you can see, when the water bear is suffering slow drying, the TDPs will upregulate, and then he will become a crystal. Magically, after rehydration, he is still alive. We chose the CAHS1 which play a important role in this process.<br>
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A separate fragment is 714bp.
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More information about our project can be found on our results page.http://2018.igem.org/Team:XMU-China/Results <br>
 
More information about our project can be found on our results page.http://2018.igem.org/Team:XMU-China/Results <br>
  

Latest revision as of 18:26, 17 October 2018


CAHS coding region

Summary

This part contains the coding region of the Hypsibius dujardini (Water bear) (Macrobiotus dujardini) CAHS gene. Cytosolic abundant heat soluble proteins acts as a molecular shield in water-deficient condition. Tardigrade-specific intrinsically disordered proteins (TDPs) are essential for desiccation tolerance by forming non-crystalline amorphous solids upon desiccation, and this vitrified state mirrors their protective capabilities.

CAHS Fig1.png


As you can see, when the water bear is suffering slow drying, the TDPs will upregulate, and then he will become a crystal. Magically, after rehydration, he is still alive. We chose the CAHS1 which play a important role in this process.
A separate fragment is 714bp.

Fig.1 The result of enzyme identification


More information about our project can be found on our results page.http://2018.igem.org/Team:XMU-China/Results


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 294
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]