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===Summary=== | ===Summary=== | ||
| − | It is a component of the Kai circuit, which dephosphorylates the phosphorylated RpaA, preventing phosphorylated RpaA from continually activating transcription of related promoters. | + | It is a component of the Kai circuit, which dephosphorylates the phosphorylated RpaA, preventing phosphorylated RpaA from continually activating transcription of related promoters. |
A separate fragment is 2265bp. | A separate fragment is 2265bp. | ||
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===CikA's function in phosphorylation pathway=== | ===CikA's function in phosphorylation pathway=== | ||
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| − | < | + | <table><tr><th>[[Image:PKaiBC_RpaA_.gif|thumb|700px|Fig.1 Simplified model of the central circadian oscillator and output activities.[1]]]</th><th></table> |
| − | < | + | ===Reference=== |
| − | < | + | [1]Joseph S. Markson,Joseph R. Piechura,Anna M. Puszynska,Erin K. O’Shea. Circadian Control of Global Gene Expression by the Cyanobacterial Master Regulator RpaA[J]. Cell,2013,155(6).<br> |
| − | < | + | More information about our project can be found on our results page.http://2018.igem.org/Team:XMU-China/Results <br> |
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| − | More information about our project can be found on our results page.http://2018.igem.org/Team:XMU-China/Results | + | |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 19:56, 17 October 2018
CikA coding region
Summary
It is a component of the Kai circuit, which dephosphorylates the phosphorylated RpaA, preventing phosphorylated RpaA from continually activating transcription of related promoters. A separate fragment is 2265bp.
Structure of CikA
CikA's function in phosphorylation pathway
Reference
[1]Joseph S. Markson,Joseph R. Piechura,Anna M. Puszynska,Erin K. O’Shea. Circadian Control of Global Gene Expression by the Cyanobacterial Master Regulator RpaA[J]. Cell,2013,155(6).
More information about our project can be found on our results page.http://2018.igem.org/Team:XMU-China/Results
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 127
Illegal BamHI site found at 2187 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]