Difference between revisions of "Part:BBa K2719013:Design"

(→‎Design Notes)
(→‎Design Notes)
 
Line 8: Line 8:
 
===Design Notes===
 
===Design Notes===
 
This was mainly created with the idea of making a control for collagen to see how a combination of reporter proteins would behave. For this we used to promoters, one for each protein (in the case of RFP it is a tetracycline repressible promoter); RFP and Blue chromoprotein coding sequences and double terminator for each one of the reporter proteins. In addition, this part was modify so it could be compatible with pSB1C3 plasmid, RFC [10] and <i>E. coli</i> BL21 (DE3).
 
This was mainly created with the idea of making a control for collagen to see how a combination of reporter proteins would behave. For this we used to promoters, one for each protein (in the case of RFP it is a tetracycline repressible promoter); RFP and Blue chromoprotein coding sequences and double terminator for each one of the reporter proteins. In addition, this part was modify so it could be compatible with pSB1C3 plasmid, RFC [10] and <i>E. coli</i> BL21 (DE3).
 +
 +
"https://static.igem.org/mediawiki/2018/2/2b/T--TecCEM--BBa_K2719013Diagram.png"
 +
<p><i>Figure 1.</i> Diagram of BBa_K2719013, double reporter system with RFP and Blue</p>
  
 
===Source===
 
===Source===

Latest revision as of 13:28, 17 October 2018


Blue Chromoprotein and RFP combined reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 938
    Illegal NheI site found at 961
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 635
    Illegal AgeI site found at 747
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This was mainly created with the idea of making a control for collagen to see how a combination of reporter proteins would behave. For this we used to promoters, one for each protein (in the case of RFP it is a tetracycline repressible promoter); RFP and Blue chromoprotein coding sequences and double terminator for each one of the reporter proteins. In addition, this part was modify so it could be compatible with pSB1C3 plasmid, RFC [10] and E. coli BL21 (DE3).

"T--TecCEM--BBa_K2719013Diagram.png"

Figure 1. Diagram of BBa_K2719013, double reporter system with RFP and Blue

Source

Blue Chromoprotein and RFP combined reporter

References