Difference between revisions of "Part:BBa K2719012:Design"

(→‎Design Notes)
(→‎Design Notes)
 
(One intermediate revision by the same user not shown)
Line 9: Line 9:
 
For tenascin was used a blue chromoprotein to improve the way of selecting colonies. For this chromoprotein was used a constitutive promoter and a double terminator. In the other case, for tenascin cassette was used a T7-lac promoter induced by IPTG. Also, it was created to be compatible with <i>E. coli</i> BL21 (DE3) and with pSB1C3 plasmid. (Figure 1)  
 
For tenascin was used a blue chromoprotein to improve the way of selecting colonies. For this chromoprotein was used a constitutive promoter and a double terminator. In the other case, for tenascin cassette was used a T7-lac promoter induced by IPTG. Also, it was created to be compatible with <i>E. coli</i> BL21 (DE3) and with pSB1C3 plasmid. (Figure 1)  
  
−
"https://static.igem.org/mediawiki/2018/9/95/T--TecCEM--BBa_K2719010Diagram.png"
+
"https://static.igem.org/mediawiki/2018/d/db/T--TecCEM--BBa_K2719012Diagram.png"
−
 
+
 
<p><i>Figure 1.</i> Diagram of BBa_K2719012, leptin expression device.</p>
 
<p><i>Figure 1.</i> Diagram of BBa_K2719012, leptin expression device.</p>
  

Latest revision as of 13:27, 17 October 2018


TCD5 coding device with blue chromoprotein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1257


Design Notes

For tenascin was used a blue chromoprotein to improve the way of selecting colonies. For this chromoprotein was used a constitutive promoter and a double terminator. In the other case, for tenascin cassette was used a T7-lac promoter induced by IPTG. Also, it was created to be compatible with E. coli BL21 (DE3) and with pSB1C3 plasmid. (Figure 1)

"T--TecCEM--BBa_K2719012Diagram.png"

Figure 1. Diagram of BBa_K2719012, leptin expression device.

Source

TCD5 coding device with blue chromoprotein

References