Difference between revisions of "Part:BBa K2623026"
(→Usage and Biology) |
(→Usage and Biology) |
||
| Line 8: | Line 8: | ||
To ensure which time point we should add the inducer IPTG, we measured the growth curve of BBa_K2623026 by detecting the OD 600 nm of E. coli(BL21) every hour. | To ensure which time point we should add the inducer IPTG, we measured the growth curve of BBa_K2623026 by detecting the OD 600 nm of E. coli(BL21) every hour. | ||
<br><table><tr><th> | <br><table><tr><th> | ||
| − | [[Image:NG6-OD.png|thumb|420px|Figure 1. The OD600 versus time of E.coli]]</th><th></table> | + | [[Image:NG6-OD.png|thumb|420px|Figure 1. The OD600 versus time of E.coli transfected with this part]]</th><th></table> |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 12:32, 17 October 2018
Archaeal ribosomal protein protein L7Ae fused with SpyCatcher.
Summary
Archaeal ribosomal protein protein L7Ae owns the ability to bind with RNA with C/Dbox RNA structure. Through the isopeptide bonds formed between SpyTag and SpyCatcher, L7Ae can be anchored with OmpA, and then secreted into OMVs. To transport our L7Ae into the periplasmic space, we insert TorA leader sequence to the circuitry. It's a non-RFP form of BBa_K2623025 and will be used to construct our total circuit.
Usage and Biology
To ensure which time point we should add the inducer IPTG, we measured the growth curve of BBa_K2623026 by detecting the OD 600 nm of E. coli(BL21) every hour.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1168
Illegal NheI site found at 1191 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]