Difference between revisions of "Part:BBa K2602021:Experience"
(→User Reviews) |
|||
| (One intermediate revision by one other user not shown) | |||
| Line 25: | Line 25: | ||
</html> | </html> | ||
===Validation of GFP fluorescence=== | ===Validation of GFP fluorescence=== | ||
| − | The experimental setup: Bacteria were grown until an OD600 of 0.2-0.4 in LB-medium and 25 ug/ml chloramphenicol. They | + | The experimental setup: Bacteria were grown until an OD600 of 0.2-0.4 in LB-medium and 25 ug/ml chloramphenicol. They were then placed in an 96-well plate in quadruplicates. The OD was measured at the beginning and at the end of the experimental time. Fluorescence was measured every 15 minutes for 16 hours and the temperature was set to 37°C. The values shown in Figure. 1 are the fluorescence intensity divided by the starting OD. Excitation was measured at 485 nm and emission at 520 nm. |
| − | [[File:T--Linkoping_Sweden--Lund.png|430px|thumb|center|Figure 1. GFP intensity over 16 hours. Error bars represent | + | [[File:T--Linkoping_Sweden--Lund.png|430px|thumb|center|Figure 1. GFP intensity over 16 hours. Error bars represent Standard Deviation. Total time is 16 hours and fluorescence was measured every 15 minutes.]] |
Latest revision as of 21:40, 17 October 2018
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K2602021
User Reviews
UNIQ19318553de737fce-partinfo-00000000-QINU UNIQ19318553de737fce-partinfo-00000001-QINU
2018 iGEM team Linkoping Sweden
2018 iGEM team Linkoping Sweden validated this part.
Validation of GFP fluorescence
The experimental setup: Bacteria were grown until an OD600 of 0.2-0.4 in LB-medium and 25 ug/ml chloramphenicol. They were then placed in an 96-well plate in quadruplicates. The OD was measured at the beginning and at the end of the experimental time. Fluorescence was measured every 15 minutes for 16 hours and the temperature was set to 37°C. The values shown in Figure. 1 are the fluorescence intensity divided by the starting OD. Excitation was measured at 485 nm and emission at 520 nm.
