Difference between revisions of "Part:BBa K2876003:Design"
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===Source=== | ===Source=== | ||
| − | BBa_K2247006. | + | BBa_K2247006. BBa_K2876001. BBa_K2876004. |
===References=== | ===References=== | ||
Latest revision as of 07:15, 17 October 2018
Lambda CL-antiAFB1-ScFv2 fusion
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 715
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1249
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part needed a 3-Alanine linker. It was codon optimized for E. coli.
Source
BBa_K2247006. BBa_K2876001. BBa_K2876004.
References
Dove, S. L., Joung, J. K., & Hochschild, A. (1997). Activation of prokaryotic transcription through arbitrary protein- protein contacts. Nature. http://doi.org/10.1038/386627a0
Pei, S.a.S., D., Construction and identification of mouse Anti-AFB1 phage single-chain antibody library. Food and Biological Engineering College, 2010.
Li, X., Li, P. and Zhang, Q., Molecular analysis of monoclonal antibodies against aflatoxins: a prediction of functional amino acid which influences antibody affinity. Chinese Academy of Agricultural Sciences, 2011.