Difference between revisions of "Part:BBa K2876003"

 
(One intermediate revision by the same user not shown)
Line 4: Line 4:
  
 
A fusion of a Lambda CL repressor and an aflatoxin sensing single chain antibody variable region (antiAFB1-ScFv2). Used in conjunction with BBa_K2876005 (https://parts.igem.org/Part:BBa_K2876005) and BBa_K2876000 (https://parts.igem.org/Part:BBa_K2876000) to create for an E. coli two hybrid system capable of detecting aflatoxin.  
 
A fusion of a Lambda CL repressor and an aflatoxin sensing single chain antibody variable region (antiAFB1-ScFv2). Used in conjunction with BBa_K2876005 (https://parts.igem.org/Part:BBa_K2876005) and BBa_K2876000 (https://parts.igem.org/Part:BBa_K2876000) to create for an E. coli two hybrid system capable of detecting aflatoxin.  
 +
 +
For our small molecule test case we chose to detect aflatoxin, a small molecule produced by Aspergillus fungi species that infects many agricultural crops, including cannabis.2,3 Aflatoxin also causes a number of health problems in people when inhaled or ingested by immunocompromised patients.7 We fused two single chain antibodies targeting aflatoxin (BBa_K2876005 and BBa_K2876003) to the lambda repressor and alpha subunit of RNA-polymerase III (BBa_K2876002) described in Dove, Joung, and Hochschild in order to create two fusion proteins capable of activating transcription in the presence of aflatoxin (Figure 4 small molecule). For our reporter we used the previously described pOL2-62 from Dove, Joung, and Hochschild fused to a RFP. See more information about our reporter below.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 02:36, 18 October 2018


Lambda CL-antiAFB1-ScFv2 fusion

A fusion of a Lambda CL repressor and an aflatoxin sensing single chain antibody variable region (antiAFB1-ScFv2). Used in conjunction with BBa_K2876005 (https://parts.igem.org/Part:BBa_K2876005) and BBa_K2876000 (https://parts.igem.org/Part:BBa_K2876000) to create for an E. coli two hybrid system capable of detecting aflatoxin.

For our small molecule test case we chose to detect aflatoxin, a small molecule produced by Aspergillus fungi species that infects many agricultural crops, including cannabis.2,3 Aflatoxin also causes a number of health problems in people when inhaled or ingested by immunocompromised patients.7 We fused two single chain antibodies targeting aflatoxin (BBa_K2876005 and BBa_K2876003) to the lambda repressor and alpha subunit of RNA-polymerase III (BBa_K2876002) described in Dove, Joung, and Hochschild in order to create two fusion proteins capable of activating transcription in the presence of aflatoxin (Figure 4 small molecule). For our reporter we used the previously described pOL2-62 from Dove, Joung, and Hochschild fused to a RFP. See more information about our reporter below.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 715
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1249
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]