Difference between revisions of "Part:BBa K2596005"
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This is a sucrose symporter protein from E. coli.  
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This is a sucrose-proton symporter from <i>E. coli</i>.  
  
 
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===Usage and Biology===
 
===Usage and Biology===
  
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CscB is a cell membrane protein that plays an integral role in transporting sucrose across the membrane as it is a sucrose-proton symporter. It is compatible in many strains of S. elongatus, including 2973, 6803 and 7942. S. elongatus is unique in that its cell membrane proton gradient is generated by maintaining a surplus of protons inside the cell. Sucrose can then be exported from the cell using the proton gradient.
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CscB is a membrane-bound sucrose-proton (hydrogen ion) symporter. This CscB is specifically codon-optimized for <i>S. elongatus</i> PCC 7942. It is compatible in many strains of <i>Synechococcus elongatus</i>, including 2973, 6803 and 7942. <i>S. elongatus</i>, like most cyanobacteria, naturally produce a basic environment. Because the symporter is hydrogen-ion dependent, sucrose flows along the gradient from inside the cell (high amount of hydrogen ions) to outside of the cell (low amount of hydrogen ions).
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Generally, it is useful to express CscB with an inducible protein as secreting sugar leads to rerouting carbon from growth [1]. As such, when using this biobrick, it's important to separate the growth and secretion phases. Because the cyanobacteria are secreting sugar, they are also more prone to contamination.
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References:
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[1] Daniel C. Ducat, J. Abraham Avelar-Rivas, Jeffrey C. Way, Pamela A. Silver. "Rerouting Carbon Flux To Enhance Photosynthetic Productivity." Appl. Environ. Microbiol. Mar 2012, 78 (8) 2660-2668; DOI: 10.1128/AEM.07901-11
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 23:50, 17 October 2018


cscB (sucrose permease) from E. coli

This is a sucrose-proton symporter from E. coli.

Usage and Biology

CscB is a membrane-bound sucrose-proton (hydrogen ion) symporter. This CscB is specifically codon-optimized for S. elongatus PCC 7942. It is compatible in many strains of Synechococcus elongatus, including 2973, 6803 and 7942. S. elongatus, like most cyanobacteria, naturally produce a basic environment. Because the symporter is hydrogen-ion dependent, sucrose flows along the gradient from inside the cell (high amount of hydrogen ions) to outside of the cell (low amount of hydrogen ions).

Generally, it is useful to express CscB with an inducible protein as secreting sugar leads to rerouting carbon from growth [1]. As such, when using this biobrick, it's important to separate the growth and secretion phases. Because the cyanobacteria are secreting sugar, they are also more prone to contamination.

References: [1] Daniel C. Ducat, J. Abraham Avelar-Rivas, Jeffrey C. Way, Pamela A. Silver. "Rerouting Carbon Flux To Enhance Photosynthetic Productivity." Appl. Environ. Microbiol. Mar 2012, 78 (8) 2660-2668; DOI: 10.1128/AEM.07901-11

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 481
    Illegal NgoMIV site found at 604
  • 1000
    COMPATIBLE WITH RFC[1000]


Sucrose Assays

For experimental methods and results on characterization, look at the page for composite part BBa_K2596015.