Difference between revisions of "Part:BBa K2876000:Experience"

(→‎Applications of BBa_K2876000)
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:
  
 
__NOTOC__
 
__NOTOC__
−
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
+
 
−
how you used this part and how it worked out.
+
To test the reporter we transformed DH5-alpha with the pOL2-62 + RFP reporter plasmid, as well as the positive control bacteriomatch lambda and alpha plasmids from Addgene (https://www.addgene.org/browse/article/8393/) and induced with IPTG. Unfortunately, when we ran the experiment on the plate reader we saw no fluorescence response (Figure 1).
 +
 
 +
 
 +
[[File:RFPgraphs.png|400px]]
 +
 
 +
Figure 1: 48hr plate reader fluorescence experiment of pOL2-62 + RFP reporter with bacteriomatch system (positive control lambda and alpha fusion proteins) and induced with IPTG.
  
 
===Applications of BBa_K2876000===
 
===Applications of BBa_K2876000===

Latest revision as of 02:56, 18 October 2018


To test the reporter we transformed DH5-alpha with the pOL2-62 + RFP reporter plasmid, as well as the positive control bacteriomatch lambda and alpha plasmids from Addgene (https://www.addgene.org/browse/article/8393/) and induced with IPTG. Unfortunately, when we ran the experiment on the plate reader we saw no fluorescence response (Figure 1).


RFPgraphs.png

Figure 1: 48hr plate reader fluorescence experiment of pOL2-62 + RFP reporter with bacteriomatch system (positive control lambda and alpha fusion proteins) and induced with IPTG.

Applications of BBa_K2876000

prokaryotic two hybrid systems

User Reviews

UNIQ0695a625a91fed71-partinfo-00000000-QINU UNIQ0695a625a91fed71-partinfo-00000001-QINU