Difference between revisions of "Part:BBa K2611002:Design"

 
 
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
This sgRNA is produced by part "sgRNA generator"(BBa_K1689000). The spacer sequence is selected from algorithm. And the backbone is BBa_K1689000.
+
This sgRNA is produced by part "sgRNA generator"(BBa_K1689000). The spacer sequence is designed by using a special algorithm. And the backbone is BBa_K1689000.
  
 
===References===
 
===References===

Latest revision as of 06:41, 17 October 2018


sgRNA (spacer J23101-RFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Should carefully notice the complementary of the sgRNA and its target sequence (part "spacer J23101-RFP"BBa_K2611003).


Source

This sgRNA is produced by part "sgRNA generator"(BBa_K1689000). The spacer sequence is designed by using a special algorithm. And the backbone is BBa_K1689000.

References