Difference between revisions of "Part:BBa K2705005:Design"
 
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===Design Notes===
 
===Design Notes===
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The part was applied to test the repression function of GltC to P<sub>''gltAB''</sub>, for which we applied different glutamate level and tested the fluorescence level changes.  
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The part was applied to test the repression function of GltC to P<sub>''gltAB''</sub>, for which we applied different glutamate level and tested the fluorescence level changes in LL3.  
  
  
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===Source===
 
===Source===
  
−
P<sub>''gltAB''</sub> is acquired from LL3 by PCR, GFP sequence is from our lab.
+
P<sub>''gltAB''</sub> was acquired from LL3 by PCR, GFP sequence was from our lab.
  
 
===References===
 
===References===

Latest revision as of 06:11, 17 October 2018


PgltAB-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 671
    Illegal XhoI site found at 572
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was applied to test the repression function of GltC to PgltAB, for which we applied different glutamate level and tested the fluorescence level changes in LL3.


Source

PgltAB was acquired from LL3 by PCR, GFP sequence was from our lab.

References