Difference between revisions of "Part:BBa K2611003"

Latest revision as of 02:16, 18 October 2018

Spacer J23101-RFP

We added a spacer sequence and a NGG site closely before the promoter (J23101). This spacer sequence can be recognized by the sgRNA we designed to direct dCas9. Once dCas9 binds with the spacer sequence, the expression of RFP will be repressed.To be used in pSB1C3.

We selected part J23101-RFP (BBa_K516132) as a reporter to test the function of the modified promoter and measure the repression level. The modified promoter does not influence the function of RFP gene as the RFP fluorescence intensity of the modified part is even stronger than the original one (Figure 3).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 30
Illegal NheI site found at 53
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 640
Illegal AgeI site found at 752
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 We added a spacer sequence and a NGG site closely before the promoter (J23101). This spacer sequence can be recognized by the sgRNA we designed to direct dCas9. Once dCas9 binds with the spacer sequence, the expression of RFP will be repressed.To be used in pSB1C3.
+We selected part J23101-RFP ([https://parts.igem.org/Part:BBa_K516132 BBa_K516132]) as a reporter to test the function of the modified promoter and measure the repression level. The modified promoter does not influence the function of RFP gene as the RFP fluorescence intensity of the modified part is even '''stronger''' than the original one (Figure 3).
+[[File:SCU_China-2018_spacer_J23101_RFP(1).png]]
+[[File:SCU_China-2018_spacer_J23101_RFP(2).png]]
+[[File:SCU_China-2018_spacer_J23101_RFP(3).png]]
 <!-- Add more about the biology of this part here