Difference between revisions of "Part:BBa K2611000"
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<partinfo>BBa_K2611000 short</partinfo> | <partinfo>BBa_K2611000 short</partinfo> | ||
| − | This sgRNA targets part "spacer J23101-GFP"(BBa_K2611001), and can combines with it's spacer sequence. This sgRNA here can combine with dCas9 protein to direct the complex to the upstream sequence of promoter of GFP to prevent transcription initiation. So, we suggest to use both this part and the GFP we have already modified ( | + | This sgRNA targets part "spacer J23101-GFP"(BBa_K2611001), and can combines with it's spacer sequence. This sgRNA here can combine with dCas9 protein to direct the complex to the upstream sequence of promoter of GFP to prevent transcription initiation. So, we suggest to use both this part and the GFP we have already modified (BBa_K2611001). |
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Latest revision as of 06:18, 17 October 2018
sgRNA (spacer J23101-GFP)
This sgRNA targets part "spacer J23101-GFP"(BBa_K2611001), and can combines with it's spacer sequence. This sgRNA here can combine with dCas9 protein to direct the complex to the upstream sequence of promoter of GFP to prevent transcription initiation. So, we suggest to use both this part and the GFP we have already modified (BBa_K2611001).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]