Difference between revisions of "Part:BBa J72009"

 
 
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<partinfo>BBa_J72009 short</partinfo>
 
<partinfo>BBa_J72009 short</partinfo>

Latest revision as of 22:06, 16 July 2008

pBca1101 RFP expression plasmid in BBb

This plasmid, referred to in-house as pBca1101 is available in Genbank/ApE format File:JCA pBca1101.txt. The plasmid is comparable to pBca9145, but it inserts an RFP open reading frame into the BamHI and XhoI sites.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 885
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 885
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 1
    Illegal BamHI site found at 29
    Illegal XhoI site found at 879
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 885
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 885
    Illegal AgeI site found at 589
    Illegal AgeI site found at 701
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1972