Difference between revisions of "Part:BBa K2832016:Design"
 
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===Design Notes===
 
===Design Notes===
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https://static.igem.org/mediawiki/parts/4/48/T--PSB1C3-_apFAB90-mRFP1_map.png
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https://static.igem.org/mediawiki/parts/7/7a/ApFAB90_Design_Diagrams.png
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Plasmid map that includes BIOFAB promoter apFAB90
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apFAB90 Test Device:  The diagram specifies the design of the engineered E. coli apFAB90 Test Cells which are transformed by the plasmid pSB1C3-BBa_K2832016.  The plasmid encodes the apFAB90 Test Device which contains the BIOFAB Promoter apFAB90.  This device was submitted for the silver medal requirement.
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https://static.igem.org/mediawiki/parts/e/e5/ApFAB90_Plasmid_Map.png
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pSB1C3-BBa_K2832016 Plasmid Map: The plasmid contains apFAB90 Test Device
  
 
===Source===
 
===Source===

Latest revision as of 20:36, 2 December 2018


BIOFAB Modular Promoter apFAB90 Driven mRFP1 Generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 629
    Illegal AgeI site found at 741
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

ApFAB90_Design_Diagrams.png apFAB90 Test Device: The diagram specifies the design of the engineered E. coli apFAB90 Test Cells which are transformed by the plasmid pSB1C3-BBa_K2832016. The plasmid encodes the apFAB90 Test Device which contains the BIOFAB Promoter apFAB90. This device was submitted for the silver medal requirement.

ApFAB90_Plasmid_Map.png pSB1C3-BBa_K2832016 Plasmid Map: The plasmid contains apFAB90 Test Device

Source

Source...

References

Mutalik,V. Guimaraes, J. Cambray, G. Lam, C. Christoffersen, M. Mai, Q. Tran, A Morgan Paull, Keasling, J. Arkin, A. Endy, D. (2013). Precise and reliable gene expression via standard transcription and translation initiation elements: Nature Methods, 354-360