Difference between revisions of "Part:BBa K2832014:Design"
 
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===Design Notes===
 
===Design Notes===
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https://static.igem.org/mediawiki/parts/f/f9/PSB1C3-_apFAB46-_mRFP1_Map.png
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https://static.igem.org/mediawiki/parts/f/f1/ApFAB46_Design_Diagram.png
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<p> Plasmid map for the apFAB46 promoter<p/>
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apFAB46 Test Device:  The diagram specifies the design of the engineered E. coli apFAB46 Test Cells which are transformed by the plasmid pSB1C3-BBa_K2832014.  The plasmid encodes the apFAB46 Test Device which contains the BIOFAB Promoter apFAB46.  This device was submitted for the silver medal requirement.
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https://static.igem.org/mediawiki/parts/e/ee/AFAB46_Plasmid_Map.png
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pSB1C3-BBa_K2832014 Plasmid Map: The plasmid contains apFAB46 Test Device
  
  

Latest revision as of 22:39, 1 December 2018


BIOFAB Modular Promoter apFAB46 Driven mRFP1 Generator ( ---> mRFP1 )


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 628
    Illegal AgeI site found at 740
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

ApFAB46_Design_Diagram.png apFAB46 Test Device: The diagram specifies the design of the engineered E. coli apFAB46 Test Cells which are transformed by the plasmid pSB1C3-BBa_K2832014. The plasmid encodes the apFAB46 Test Device which contains the BIOFAB Promoter apFAB46. This device was submitted for the silver medal requirement. AFAB46_Plasmid_Map.png pSB1C3-BBa_K2832014 Plasmid Map: The plasmid contains apFAB46 Test Device


Source

Source...

References

Mutalik,V. Guimaraes, J. Cambray, G. Lam, C. Christoffersen, M. Mai, Q. Tran, A Morgan Paull, Keasling, J. Arkin, A. Endy, D. (2013). Precise and reliable gene expression via standard transcription and translation initiation elements: Nature Methods, 354-360