Difference between revisions of "Part:BBa K1829002"
 
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===Part Verification===
 
===Part Verification===
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iGEM18_NEFU_China designed the experiment and carried out the verification of the promoter. We induced the activation of pFig2c by the alpha factor we extracted, and the expression of eGFP protein was observed with fluorescence (Fig.1). The negative control (Fig.2, without alpha factor) showed no fluorescence.
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iGEM18_NEFU_China designed the experiment and carried out the verification of the promoter. We induced the activation of pFig2c by the α factor we extracted, and the expression of EGFP protein was observed with fluorescence (Fig.1 add with α factor). The negative control (Fig.2, without α factor) showed no fluorescence. [[http://2018.igem.org/Team:NEFU_China/Suicide See more details]]
 
So this part is successfully verified.
 
So this part is successfully verified.
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[[Image:T--NEFU_China--BBa_K1829002_Fig1.png|thumb|Fig.1]]
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[[Image:T--NEFU_China--BBa_K1829002_Figure.png|thumb|Fig.1 add with α factor.Fig.2 without α factor.|560px]]
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[[Image:T--NEFU_China--BBa_K1829002_Fig2.png|thumb|Fig.2]]
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Latest revision as of 08:43, 17 October 2018


pFig2c: Mating factor alpha responsive promoter

pFig2c is a mating factor alpha responsive promoter, meaning that it is activated by the presence of mating factor alpha. It is endogenous in S. Cerevisiae cells and is followed by the Fig2c gene. The Fig2c gene is a part of the mating pathway in S. Cerevisiae, it contributes to maintaining cell wall integrity when the cells project ("shmoo") towards each other.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 431
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 125
    Illegal BsaI.rc site found at 564


Part Verification

iGEM18_NEFU_China designed the experiment and carried out the verification of the promoter. We induced the activation of pFig2c by the α factor we extracted, and the expression of EGFP protein was observed with fluorescence (Fig.1 add with α factor). The negative control (Fig.2, without α factor) showed no fluorescence. http://2018.igem.org/Team:NEFU_China/Suicide See more details So this part is successfully verified.

Fig.1 add with α factor.Fig.2 without α factor.