Difference between revisions of "Part:BBa K2832006"

Latest revision as of 23:38, 17 October 2018

RcsB/NhaR mRFP1 Generator ( RcsB/NhaR ---> OsmCp ---> mRFP1 ) RcsB and NhaR are transcription factors that bind to osmC promoter sequence. rcsB is responsible for cell division, periplasmic proteins, motility, and biofilm formation. rcsB described as “global regulator controlling cell surface composition in response to changing environment.” Sound could play role in antagonizing one of these systems to activate rcsB. NhaR is dependent on Na+ and controls genes for defense against Na+, alkaline pH environments, adverse conditions, and biofilm formation. Again this represented a large array of systems that sound may antagonize. This potential sound sensitive promoter is attached to the sequence that codes for RFP. When the promoter is activated it stimulates the expression of RFP.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 13
Illegal AgeI site found at 829
Illegal AgeI site found at 941
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K2832006 short</partinfo>
-RcsB and NhaR are transcription factors that bind to osmC promoter sequence. rcsB is responsible for cell division, periplasmic proteins, motility, and biofilm formation. rcsB described as “global regulator controlling cell surface composition in response to changing environment.” Sound could play role in antagonizing one of these systems to activate rcsB. NhaR is dependent on Na+ and controls genes for defense against Na+, alkaline pH environments, adverse conditions, and biofilm formation. Again this represented a large array of systems that sound may antagonize.
+RcsB and NhaR are transcription factors that bind to osmC promoter sequence. rcsB is responsible for cell division, periplasmic proteins, motility, and biofilm formation. rcsB described as “global regulator controlling cell surface composition in response to changing environment.” Sound could play role in antagonizing one of these systems to activate rcsB. NhaR is dependent on Na+ and controls genes for defense against Na+, alkaline pH environments, adverse conditions, and biofilm formation. Again this represented a large array of systems that sound may antagonize. This potential sound sensitive promoter is attached to the sequence that codes for RFP. When the promoter is activated it stimulates the expression of RFP.
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