Difference between revisions of "Part:BBa K115004:Design"

 
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===Design Notes===
 
===Design Notes===
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The secondary structure is important to the function of these regions, but part of the wt secondary structure is destroyed by the scar. We've tried to alter the sequence so the predicted structure (through mfold and those kind of servers) is sort of conserved, but temperature sensitivity still has to be tested. If it doesn't work, possible solution might be the addition of a larger conserved part of the wt, which implies a small part of wt protein sequence as well.
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The 3D folding is important to the function of this region, but part of the structure would be destroyed by the scar when the part would contain only the temparature sensitive region. Therefor a tag is added behind this region. (we still have to determine what tag would be appropriate or if another solution would be better).
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===Source===
 
===Source===

Latest revision as of 09:51, 16 July 2008

A temperature sensitive 5


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The 3D folding is important to the function of this region, but part of the structure would be destroyed by the scar when the part would contain only the temparature sensitive region. Therefor a tag is added behind this region. (we still have to determine what tag would be appropriate or if another solution would be better).

Source

The sequence is based on the paper "RNA Quadruplex-Based Modulation of Gene Expression" by Markus Wieland and Jorg S. Hartig.

References