Difference between revisions of "Part:BBa K2729013"
 
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The prM protein consists of an N-terminal pr domain followed by the M protein separated by a furin cleavage site.(15) The pr part of the protein consists mainly of β-strands, whereas the M portion consists of a linear structure followed by a mainly α-helical stem region and two transmembrane helices.
 
The prM protein consists of an N-terminal pr domain followed by the M protein separated by a furin cleavage site.(15) The pr part of the protein consists mainly of β-strands, whereas the M portion consists of a linear structure followed by a mainly α-helical stem region and two transmembrane helices.
  
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[[File:Tokyotech 2018 EP (E,prME).png.png|thumb|left|300px| '''Figure 1:''' '''Result of the Electrophoresis''' ]]
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[[File:Tokyotech 2018 EP (E,prME).png|thumb|left|300px| '''Figure 1:''' '''Result of the Electrophoresis''' ]]
  
 
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As shown in Figure 1, the band of DENV4 prME come around 2,000 bp.  
 
As shown in Figure 1, the band of DENV4 prME come around 2,000 bp.  
 
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<br style="clear: both" />
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===Analysis===
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Our team successfully validated that the sequence was synthesized, and obtained enough amount of it for the transfection.
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If it works well in the process of pseudo-virus production and also infection into host cells as expected, the fluorescence intensity can be observed robustly.
  
 
==Sequence and Features==
 
==Sequence and Features==

Latest revision as of 03:34, 18 October 2018


DENV4 ancC-prM-E (Dengue Virus Serotype IV)

Usage and Biology

Flavivirus Capsid (C), prM (precursor Membrane) and Envelope (E) are structural proteins responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.

Characterization

The prM protein consists of an N-terminal pr domain followed by the M protein separated by a furin cleavage site.(15) The pr part of the protein consists mainly of β-strands, whereas the M portion consists of a linear structure followed by a mainly α-helical stem region and two transmembrane helices.

Figure 1: Result of the Electrophoresis


As shown in Figure 1, the band of DENV4 prME come around 2,000 bp.

Analysis

Our team successfully validated that the sequence was synthesized, and obtained enough amount of it for the transfection.

If it works well in the process of pseudo-virus production and also infection into host cells as expected, the fluorescence intensity can be observed robustly.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 488
    Illegal EcoRI site found at 1947
    Illegal SpeI site found at 1219
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 488
    Illegal EcoRI site found at 1947
    Illegal SpeI site found at 1219
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 488
    Illegal EcoRI site found at 1947
    Illegal BamHI site found at 1910
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 488
    Illegal EcoRI site found at 1947
    Illegal SpeI site found at 1219
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 488
    Illegal EcoRI site found at 1947
    Illegal SpeI site found at 1219
    Illegal NgoMIV site found at 1207
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 793

Reference

- Membranotropic regions of the dengue virus prM protein