Difference between revisions of "Part:BBa J72003:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_J72003 short</partinfo> | <partinfo>BBa_J72003 short</partinfo> | ||
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===Source=== | ===Source=== | ||
| − | + | <pre> | |
| − | + | PCR ca1182F/ca1182R on pG80 CRIM plasmid (BglII/XhoI) | |
| + | Sub into pBca9145-Bca1144#5 (BglII/XhoI,L) | ||
| + | Product is pBca9145-Bca1182 | ||
| + | ---- | ||
| + | ca1182F BBb Biobricking of R6K | ||
| + | ctctgAGATCTaGCAGTTCAACCTGTTGATAG | ||
| + | ca1182R BBb biobricking of R6K | ||
| + | gcaaaCTCGAGttaGGATCCgtgattcgcacgggcccatg | ||
| + | </pre> | ||
===References=== | ===References=== | ||
Latest revision as of 05:26, 16 July 2008
{R6K} conditional origin of replication in BBb
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
N/A
Source
PCR ca1182F/ca1182R on pG80 CRIM plasmid (BglII/XhoI) Sub into pBca9145-Bca1144#5 (BglII/XhoI,L) Product is pBca9145-Bca1182 ---- ca1182F BBb Biobricking of R6K ctctgAGATCTaGCAGTTCAACCTGTTGATAG ca1182R BBb biobricking of R6K gcaaaCTCGAGttaGGATCCgtgattcgcacgggcccatg