Difference between revisions of "Part:BBa K2680268:Design"
 
Line 7: Line 7:
 
===Design Notes===
 
===Design Notes===
  
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Sequence sent to William and Mary iGEM from Murray Lab a the California Institute of Technology. Used PCR to add William and Mary Pad overhangs, which were used to clone the part into William and Mary Pad backbone. Forbidden restriction sites were checked for and removed if necessary.
+
Sequence sent to William and Mary iGEM from Murray Lab at the California Institute of Technology. Used PCR to add William and Mary Pad overhangs, which were used to clone the part into William and Mary Pad backbone. Forbidden restriction sites were checked for and removed if necessary.
  
 
===Source===
 
===Source===

Latest revision as of 00:22, 17 October 2018


3G YFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 47
    Illegal BsaI.rc site found at 786

Design Notes

Sequence sent to William and Mary iGEM from Murray Lab at the California Institute of Technology. Used PCR to add William and Mary Pad overhangs, which were used to clone the part into William and Mary Pad backbone. Forbidden restriction sites were checked for and removed if necessary.

Source

"Yellow Fluorescent Protein derived from Aequorea victoria GFP. This sequence is cloned from the pZE12-YFP plasmid used by Elowitz (see reference). The original gene was made by the The Yeast Resource Center (YRC) based at the University of Washington in Seattle, Washington. [...] This part is useful as a reporter."1

References

1Lundin, E. (2011, August 21). Part:BBa_K592101. Retrieved October 16, 2018, from https://parts.igem.org/Part:BBa_K592101