Difference between revisions of "Part:BBa K2558216:Design"
 
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=NEON CRISPRi Safety Catch device with lux pR-HS promotor=
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__NOTOC__
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<partinfo>BBa_K2558216 short</partinfo>
  
 
<partinfo>BBa_K2558216 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2558216 SequenceAndFeatures</partinfo>
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===Design Note===
 
===Design Note===
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In order to investigate how lacI dosage affect IPTG induction we used Anderson promotor J23100, J23110 and J23114 to design three constitutive lacI generator of different intensity. The three lacI generator were then ligated with Ptac controlled reporter sfGFP to make three IPTG induction devices (https://parts.igem.org/Part:BBa_K2558203, https://parts.igem.org/Part:BBa_K2558204, https://parts.igem.org/Part:BBa_K2558205). By measuring sfGFP fluorescence we tested how these devices react to IPTG.
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Assisted with the experiences we gained form the experiments above, we built and tuned the NEON system. We designed this experiment to characterize how Neon the positive feedback plasmid (BBa_K2558214), and Safety Catch the CRISPRi plasmid (BBa_K2558215, BBa_K2558216) work together.
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===Source===
 
===Source===

Latest revision as of 16:46, 17 October 2018

CRISPRi Safety Catch device with lux pR-HS promotor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 310
    Illegal NheI site found at 333
    Illegal NheI site found at 1466
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5850
    Illegal BamHI site found at 3745
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 79
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4617


Design Note

Assisted with the experiences we gained form the experiments above, we built and tuned the NEON system. We designed this experiment to characterize how Neon the positive feedback plasmid (BBa_K2558214), and Safety Catch the CRISPRi plasmid (BBa_K2558215, BBa_K2558216) work together.

Source

References