Difference between revisions of "Part:BBa K2560011:Design"
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The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector <a href="https://parts.igem.org/Part:BBa_K2560002">BBa_K2560002</a> using Golden Gate assembly. If you stuggle with <i> de novo </i> synthesis we recomended this | The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector <a href="https://parts.igem.org/Part:BBa_K2560002">BBa_K2560002</a> using Golden Gate assembly. If you stuggle with <i> de novo </i> synthesis we recomended this | ||
<a href="https://parts.igem.org/Help:Promoters/Construction">site</a>. | <a href="https://parts.igem.org/Help:Promoters/Construction">site</a>. | ||
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</html> | </html> | ||
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<b> Forward Oligo:</b> | <b> Forward Oligo:</b> | ||
− | + | CTCGAACAGAATTCGCGGCCGCTTCTAGAGGGAG | |
+ | |||
<b> Reverse Oligo:</b> | <b> Reverse Oligo:</b> | ||
− | + | CTCACTCCCTCTAGAAGCGGCCGCGAATTCTGTT |
Latest revision as of 00:02, 17 October 2018
Phytobrick version of 5'Connector Dummy
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal NotI site found at 7 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
- 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal XbaI site found at 16 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This connectors were designed to enable flexible cloning of LVL 2 plasmids. For more informations feel free to visit our Design Page.
Source
The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly. If you stuggle with de novo synthesis we recomended this site.
Forward Oligo: CTCGAACAGAATTCGCGGCCGCTTCTAGAGGGAG
Reverse Oligo: CTCACTCCCTCTAGAAGCGGCCGCGAATTCTGTT