Difference between revisions of "Part:BBa K2812003:Design"
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The initial starting sequence for truncated lysostaphin was taken from <partinfo>BBa_K748002</partinfo>. This amino acid sequence was used to generate a codon optimized sequence for expression in E. coli. | The initial starting sequence for truncated lysostaphin was taken from <partinfo>BBa_K748002</partinfo>. This amino acid sequence was used to generate a codon optimized sequence for expression in E. coli. | ||
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===Source=== | ===Source=== | ||
| − | + | The designed sequence was synthesized ''de novo'' by IDT. | |
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Latest revision as of 22:35, 16 October 2018
Coding sequence for trunctated Lysostaphin regulated by T7-promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 164
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The initial starting sequence for truncated lysostaphin was taken from BBa_K748002. This amino acid sequence was used to generate a codon optimized sequence for expression in E. coli.
Source
The designed sequence was synthesized de novo by IDT.