Difference between revisions of "Part:BBa K2680263:Design"
 
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===References===
 
===References===
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<sup>1</sup>
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<sup>1</sup>Kj, A. (2013, August 20). Part:BBa_K1088005. Retrieved October 16, 2018, from https://parts.igem.org/Part:BBa_K1088005

Latest revision as of 21:14, 16 October 2018


3G AraC-ssrA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 47
    Illegal BsaI.rc site found at 978


Design Notes

Sequence sent to William and Mary iGEM from Murray Lab at California Institute of Technology. Used PCR to add William and Mary Pad overhangs, which were used to clone the part into William and Mary Pad backbone. Forbidden restriction sites were checked for and removed if necessary.

Source

"E. coli K-12 MG1655"1

References

1Kj, A. (2013, August 20). Part:BBa_K1088005. Retrieved October 16, 2018, from https://parts.igem.org/Part:BBa_K1088005