Difference between revisions of "Part:BBa K209023"
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===2018 Peking Team improvement===
 
===2018 Peking Team improvement===
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Team Peking 2018 improved this part.
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Team Peking 2018 improved this part. Please view http://2018.igem.org/Team:Peking/Improve for more details.
 +
 
 +
Firstly, we fused this this part with yEGFP, which made it visible in the yeast under fluorescent microscope.
  
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First, we fuse this this part with yEGFP, which make it visible in the yeast under fluorescent microscope.
 
 
https://parts.igem.org/Part:BBa_K2601008
 
https://parts.igem.org/Part:BBa_K2601008
  
−
Then, we drive the expression of FKBP-yEGFP with 3 promoters pTet07,pTEF1 and PDH3. The expression of these promoters were measure by flow cytometry.
+
Then we characterized the expression of FKBP-yEGFP with 3 promoters pTet07, pTEF1 and PDH3. The expression of these promoters was measured through flow cytometry.
 +
 
 +
https://parts.igem.org/Part:BBa_K2601025;
 +
https://parts.igem.org/Part:BBa_K2601026;
 +
https://parts.igem.org/Part:BBa_K2601027
 +
 
 +
Moreover, we fused FKBP-yEGFP with HOtag3. This part can form phase separation with Frb-yEGFP-HOTag6 in the presence of rapamycin.
  
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href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601025
+
https://parts.igem.org/Part:BBa_K2601011
−
href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601026
+
−
href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601027
+
  
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What's more, we fuse FKBP-yEGFP with HOtag3. This part can form phase separation with Frb-yEGFP-HOTag6 in the presence of rapamycin.
+
Finally, we characterized the expression of FKBP-yEGFP-HOTag3 with 2 promoters pTEF1 and PDH3. The expression of these promoters was measured by flow cytometry.
−
<a href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601011</a><br/>
+
  
−
Then, we drive the expression of FKBP-yEGFP-HOTag6 with 2 promoters pTEF1 and PDH3. The expression of these promoters were measure by flow cytometry.
+
https://parts.igem.org/Part:BBa_K2601037;
−
<a href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601037</a><br/>
+
https://parts.igem.org/Part:BBa_K2601038
−
<a href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601038</a><br/>
+

Latest revision as of 11:00, 17 October 2018


Aar1 C-D part, FKBP

dimerizes with FRB when rapamycin is present

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


2018 Peking Team improvement

Team Peking 2018 improved this part. Please view http://2018.igem.org/Team:Peking/Improve for more details.

Firstly, we fused this this part with yEGFP, which made it visible in the yeast under fluorescent microscope.

https://parts.igem.org/Part:BBa_K2601008

Then we characterized the expression of FKBP-yEGFP with 3 promoters pTet07, pTEF1 and PDH3. The expression of these promoters was measured through flow cytometry.

https://parts.igem.org/Part:BBa_K2601025; https://parts.igem.org/Part:BBa_K2601026; https://parts.igem.org/Part:BBa_K2601027

Moreover, we fused FKBP-yEGFP with HOtag3. This part can form phase separation with Frb-yEGFP-HOTag6 in the presence of rapamycin.

https://parts.igem.org/Part:BBa_K2601011

Finally, we characterized the expression of FKBP-yEGFP-HOTag3 with 2 promoters pTEF1 and PDH3. The expression of these promoters was measured by flow cytometry.

https://parts.igem.org/Part:BBa_K2601037; https://parts.igem.org/Part:BBa_K2601038