Difference between revisions of "Part:BBa K2665025"
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<partinfo>BBa_K2665025 short</partinfo> | <partinfo>BBa_K2665025 short</partinfo> | ||
− | SED1 secretion signal is signal peptide sequence derived from <i>S.cerevisiae</i> SED1 gene.<br> | + | SED1 secretion signal is a signal peptide sequence derived from <i>S.cerevisiae</i> SED1 gene.<br> |
− | This signal peptide sequence | + | This signal peptide sequence can be utilized for cell-surface display of various proteins. [1]<br> |
− | Using this part with SED1 anchoring domain, you can anchor objective protein on | + | Using this part with the SED1 anchoring domain ''' [https://parts.igem.org/Part:BBa_K2665026 BBa_K2665026] ''', you can anchor your objective protein on S.cerevisiae cell wall.<br> |
− | + | The usage example of this part can be referred in the pages of ''' [https://parts.igem.org/Part:BBa_K2665020 BBa_K2665020] ''' ''' [https://parts.igem.org/Part:BBa_K2665021 BBa_K2665021] ''' | |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 01:13, 17 October 2018
SED1 secretion signal
SED1 secretion signal is a signal peptide sequence derived from S.cerevisiae SED1 gene.
This signal peptide sequence can be utilized for cell-surface display of various proteins. [1]
Using this part with the SED1 anchoring domain BBa_K2665026 , you can anchor your objective protein on S.cerevisiae cell wall.
The usage example of this part can be referred in the pages of BBa_K2665020 BBa_K2665021
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Reference
[1]K. Inokuma, T.Bamba, J.Ishii et al. (2016) "Enhanced cell-surface display and secretory production of cellulolytic enzymes with Saccharomyces cerevisiae Sed1 signal peptide" Biotechnology and Bioengineering 2358-2366