Difference between revisions of "Part:BBa K2587029:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | We performed a mutagenesis of recognition sites from the restriction enzymes BsaI and BbsI. | + | We performed a mutagenesis of recognition sites from the restriction enzymes BsaI and BbsI in the coding sequence of <i>ddh</i>. |
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===Source=== | ===Source=== | ||
− | The gene ddh was isolated from the genome of <i>Corynebacterium glutamicum</i> and the other parts are taken from the CIDAR MoClo toolbox. | + | The gene <i>ddh</i> was isolated from the genome of <i>Corynebacterium glutamicum</i> and the other parts are taken from the CIDAR MoClo toolbox. |
===References=== | ===References=== |
Latest revision as of 14:07, 16 October 2018
Pj23102_RBS_ddh_T
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 422
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 390
Illegal NgoMIV site found at 615
Illegal AgeI site found at 825 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We performed a mutagenesis of recognition sites from the restriction enzymes BsaI and BbsI in the coding sequence of ddh.
Source
The gene ddh was isolated from the genome of Corynebacterium glutamicum and the other parts are taken from the CIDAR MoClo toolbox.