Difference between revisions of "Part:BBa K2560011:Design"
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===Design Notes===
 
===Design Notes===
 
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The sequence was synthesized <i> in silico </i> by Daniel Stukenberg and ordered from <a href="https://eu.idtdna.com/pages">Integrated DNA Technologies</a>(IDT).  
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This connectors were designed to enable flexible cloning of LVL 2 plasmids. For more informations feel free to visit our <a href="http://2018.igem.org/Team:Marburg/Design">Design Page</a>.
 
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===Source===
 
===Source===
 
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The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector <a href="https://parts.igem.org/Part:BBa_K2560002">BBa_K2560002</a> using Golden Gate assembly. If you stuggle with <i> de novo </i> synthesis we recomended this
 
The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector <a href="https://parts.igem.org/Part:BBa_K2560002">BBa_K2560002</a> using Golden Gate assembly. If you stuggle with <i> de novo </i> synthesis we recomended this
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<a href="https://parts.igem.org/Help:Promoters/Construction">site</a>.
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<a href="https://parts.igem.org/Help:Promoters/Construction">site</a>.  
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<b> Forward Oligo:</b>
 
<b> Forward Oligo:</b>
 
CTCGAACAGAATTCGCGGCCGCTTCTAGAGGGAG
 
CTCGAACAGAATTCGCGGCCGCTTCTAGAGGGAG
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<b> Reverse Oligo:</b>
 
<b> Reverse Oligo:</b>
 
CTCACTCCCTCTAGAAGCGGCCGCGAATTCTGTT
 
CTCACTCCCTCTAGAAGCGGCCGCGAATTCTGTT
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===References===
 

Latest revision as of 00:02, 17 October 2018


Phytobrick version of 5'Connector Dummy


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NotI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

This connectors were designed to enable flexible cloning of LVL 2 plasmids. For more informations feel free to visit our Design Page.

Source

The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly. If you stuggle with de novo synthesis we recomended this site.

Forward Oligo: CTCGAACAGAATTCGCGGCCGCTTCTAGAGGGAG

Reverse Oligo: CTCACTCCCTCTAGAAGCGGCCGCGAATTCTGTT