Difference between revisions of "Part:BBa K2680261"

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<partinfo>BBa_K2680261 short</partinfo>
 
<partinfo>BBa_K2680261 short</partinfo>
  
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3G sfYFP
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3G sfYFP gene flanked by prefix sticky end C and suffix sticky end D in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].
  
 
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===References===
 
===References===
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[1] Frenzel, E., Legebeke, J., van Stralen, A., van Kranenburg, R., & Kuipers, O. P. (2018). In vivo selection of sfGFP variants with improved and reliable functionality in industrially important thermophilic bacteria. Biotechnology for Biofuels, 11, 8. http://doi.org/10.1186/s13068-017-1008-5
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[1] https://parts.igem.org/Part:BBa_K1093001

Latest revision as of 15:42, 16 October 2018


3G sfYFP

3G sfYFP gene flanked by prefix sticky end C and suffix sticky end D in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 47
    Illegal BsaI.rc site found at 783
    Illegal SapI.rc site found at 70


Description

Superfolding yellow fluorescent protein with an excitation maximum of 503nm and emission maximum of 540nm as characterized by team Warsaw_2013[1]

References

[1] https://parts.igem.org/Part:BBa_K1093001