Difference between revisions of "Part:BBa K2680281"
 
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<partinfo>BBa_K2680281 short</partinfo>
 
<partinfo>BBa_K2680281 short</partinfo>
  
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3G LsRK
 
  
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"LsrK is a kinase involved in a LuxS/AI-2 signaling pathway in Salmonella Typhimurium. This kinase catalyzes the phosphorolation of autoinducer-2 (AI-2) to phospho-AI-2. AI-2 acts as a "universal signal" for interspecies communication for quorum sensing. "<sup>1</sup>
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3G LsRK is flanked by prefix sticky end C and suffix sticky end D in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].
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LsrK is a kinase involved in a LuxS/AI-2 signaling pathway in Salmonella Typhimurium. This kinase catalyzes the phosphorylation of autoinducer-2 (AI-2) to phospho-AI-2. AI-2 acts as a "universal signal" for interspecies communication for quorum sensing.<sup>1</sup>
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William and Mary iGEM encountered the coding sequence for LsRK through our collaboration with Virginia iGEM. For more information, visit [http://2018.igem.org/Team:Virginia/Parts Virginia iGEM's wiki]<sup>1</sup>
  
 
===References===
 
===References===
 
<sup>1</sup>  
 
<sup>1</sup>  
 
Wang, H., & I. (2015, September 6). Part:BBa_K1666004. Retrieved from https://parts.igem.org/Part:BBa_K1666004
 
Wang, H., & I. (2015, September 6). Part:BBa_K1666004. Retrieved from https://parts.igem.org/Part:BBa_K1666004
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Also see Zhu, J., Hixon, M. S., Globisch, D., Kaufmann, G. F., & Janda, K. D. (2013, May 29). Mechanistic insights into the LsrK kinase required for autoinducer-2 quorum sensing activation. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/23672516
  
 
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Latest revision as of 16:30, 15 October 2018


3G LsRK


3G LsRK is flanked by prefix sticky end C and suffix sticky end D in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].

LsrK is a kinase involved in a LuxS/AI-2 signaling pathway in Salmonella Typhimurium. This kinase catalyzes the phosphorylation of autoinducer-2 (AI-2) to phospho-AI-2. AI-2 acts as a "universal signal" for interspecies communication for quorum sensing.1

William and Mary iGEM encountered the coding sequence for LsRK through our collaboration with Virginia iGEM. For more information, visit [http://2018.igem.org/Team:Virginia/Parts Virginia iGEM's wiki]1

References

1 Wang, H., & I. (2015, September 6). Part:BBa_K1666004. Retrieved from https://parts.igem.org/Part:BBa_K1666004

Also see Zhu, J., Hixon, M. S., Globisch, D., Kaufmann, G. F., & Janda, K. D. (2013, May 29). Mechanistic insights into the LsrK kinase required for autoinducer-2 quorum sensing activation. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/23672516

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 41
    Illegal BsaI.rc site found at 1647