Difference between revisions of "Part:BBa K124000:Experience"

 
(→‎Applications of BBa_K124000)
 
Line 5: Line 5:
  
 
===Applications of BBa_K124000===
 
===Applications of BBa_K124000===
 +
 +
 +
Activation and Repression of CYC1 using scRNAs
 +
 +
 +
[[File:T--EPFL--CYC_CHARACTERIZATION.png|200px|thumb|left|CYC1_Characterization]]
 +
 +
 +
Expression control of CYC1 promoter in yeasts.
 +
A histogram shows fluorescence intensities of GFP when it is produced under basal level, activation or repression. The basal level represents cells single-transfected with CYC1-GFP. For the activation, the strain was additionally transfected with dCas9, scCyc1_MS2 and the activation module MCP-VP64. For the repression, the scaffold RNA is scCyc1_PP7 and the transcriptional module is PCP-Mxi1. MS2 targets region c3 of the CYC1 promoter, while PP7 targets region c6. Then, with these basic elements, in principle all gates could be modularly assembled.
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 00:34, 20 October 2016


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K124000

Activation and Repression of CYC1 using scRNAs


CYC1_Characterization


Expression control of CYC1 promoter in yeasts. A histogram shows fluorescence intensities of GFP when it is produced under basal level, activation or repression. The basal level represents cells single-transfected with CYC1-GFP. For the activation, the strain was additionally transfected with dCas9, scCyc1_MS2 and the activation module MCP-VP64. For the repression, the scaffold RNA is scCyc1_PP7 and the transcriptional module is PCP-Mxi1. MS2 targets region c3 of the CYC1 promoter, while PP7 targets region c6. Then, with these basic elements, in principle all gates could be modularly assembled.

User Reviews

UNIQ06788e2f355d0c71-partinfo-00000000-QINU UNIQ06788e2f355d0c71-partinfo-00000001-QINU