Difference between revisions of "Part:BBa K2582002"
 
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<partinfo>BBa_K2582002 short</partinfo>
 
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This part is the T7 RNA Polymerase-based expression cassette of the RNA Aptamer Probe (RAP)  [https://parts.igem.org/Part:BBa_K2582001 BBa_K2582001]. It targets the influenza Neuraminidase subtype 9 (Gene accession number: CY235364.1) by nucleotide 545-566.
 
This part is the T7 RNA Polymerase-based expression cassette of the RNA Aptamer Probe (RAP)  [https://parts.igem.org/Part:BBa_K2582001 BBa_K2582001]. It targets the influenza Neuraminidase subtype 9 (Gene accession number: CY235364.1) by nucleotide 545-566.
  
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===Usage and Biology===
 
===Usage and Biology===
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This part is modified from the Spinach aptamer, the RNA molecule that can bind and increase the quantum yield of
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<p>This part is assembled for the expression of a RNA Aptamer Probe, a RNA aptamer that enhances the fluorescence of its docking fluorogen upon binding of a complementary RNA. When transformed into any DE3 strains of <i>E. coli</i>, it can be expressed by IPTG induction. However, upon expression of the RNA aptamer probe and the target RNA, no fluorescence is observed.</p>
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[[File:Total RNA.jpg|600px|thumb|left|Fluorescence data obtained from plate reader. Total RNA is extracted using RNA-Snap technique while its quality is verified by agarose gel electrophoresis. N=1. More replicates are needed.]]
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 12:29, 16 October 2018

T7 Promoter-RAP(N9-545-562)-T7 Terminator

This part is the T7 RNA Polymerase-based expression cassette of the RNA Aptamer Probe (RAP) BBa_K2582001. It targets the influenza Neuraminidase subtype 9 (Gene accession number: CY235364.1) by nucleotide 545-566.

Usage and Biology

This part is assembled for the expression of a RNA Aptamer Probe, a RNA aptamer that enhances the fluorescence of its docking fluorogen upon binding of a complementary RNA. When transformed into any DE3 strains of E. coli, it can be expressed by IPTG induction. However, upon expression of the RNA aptamer probe and the target RNA, no fluorescence is observed.

Fluorescence data obtained from plate reader. Total RNA is extracted using RNA-Snap technique while its quality is verified by agarose gel electrophoresis. N=1. More replicates are needed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 90
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]