Difference between revisions of "Part:BBa K2541406:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The secondary structure is important to the function of an RNA thermosensor. We designed the thermosensor according to the stem length, loop size and | + | The secondary structure is important to the function of an RNA-based thermosensor. We designed the thermosensor according to the stem length, loop size and mismatches or bulges in the stem. |
===Source=== | ===Source=== | ||
| − | The thermosensor sequence is not derived from any organism. We designed it on our own and synthesized this sequence from a synthesis company. Other sequences are derived from iGEM Registry of Standard Biological Parts. | + | The thermosensor sequence is not derived from any organism. We designed it on our own and synthesized this sequence from a synthesis company. sfGFP_optimism sequence was designed by Overkamp W et al at 2013.Other sequences are derived from iGEM Registry of Standard Biological Parts. |
===References=== | ===References=== | ||
Latest revision as of 07:11, 14 October 2018
Heat-repressible RNA-based thermosensor measurement device
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 539
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 74
Design Notes
The secondary structure is important to the function of an RNA-based thermosensor. We designed the thermosensor according to the stem length, loop size and mismatches or bulges in the stem.
Source
The thermosensor sequence is not derived from any organism. We designed it on our own and synthesized this sequence from a synthesis company. sfGFP_optimism sequence was designed by Overkamp W et al at 2013.Other sequences are derived from iGEM Registry of Standard Biological Parts.
References
[1]Hoynes-O'Connor A, Hinman K, Kirchner L, et al. De novo design of heat-repressible RNA thermosensors in E. coli[J]. Nucleic Acids Research, 2015, 43(12):6166-6179.
[2]Kortmann J, Narberhaus F. Bacterial RNA thermometers: molecular zippers and switches.[J]. Nature Reviews Microbiology, 2012, 10(4):255-65.
[3]Pédelacq J-D, Cabantous S, Tran T, Terwilliger TC, Waldo GS. 2006. Engineering and characterization of a superfolder green fluorescent protein. Nat. Biotechnol. 24:79 –88.
[4]Overkamp W, Beilharz K, Detert O W R, et al. Benchmarking various green fluorescent protein variants in Bacillus subtilis, Streptococcus pneumoniae, and Lactococcus lactis for live cell imaging.[J]. Applied & Environmental Microbiology, 2013, 79(20):6481-6490.