Difference between revisions of "Part:BBa K091001"

 
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<partinfo>BBa_K091001 short</partinfo>
 
<partinfo>BBa_K091001 short</partinfo>
  
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This sequence codes for the LsrR gene which is found in the E. coli strain MG1655. When this protein fors a complex with phosphorylated AI-2 it promotes the LsrA promoter.  
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This gene codes for the LsrR protein from E. coli strain MG1655. The protein is an repressor of the pLsrA promoter that can be derepressed upon binding to phosphorylated AI-2 signal molecule. 
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===Information contributed by City of London UK (2021)===
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Part information is collated here to help future users of the BioBrick registry.
  
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Metadata:
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===Usage and Biology===
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*'''Group:''' City of London UK 2021
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*'''Author:''' Lucas Ng
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*'''Summary:''' Added information collated from existing scientific studies
  
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K091001 SequenceAndFeatures</partinfo>
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The AI-2 signal quorum-sensing signal molecule is actively transported into the cell by the LsrABC protein, before being phosphorylated by LsrK. Subsequently, Phospho-AI-2 derepresses LsrR from the operator region of the lsr operon, as stated above. In the absence of AI-2, both the lsrACDBFG operon and LsrR itself are repressed <ref>Li, Jun, Can Attila, Liang Wang, Thomas K. Wood, James J. Valdes, and William E. Bentley. 2007. “Quorum Sensing in Escherichia Coli Is Signaled by AI-2/LsrR: Effects on Small RNA and Biofilm Architecture.” Journal of Bacteriology 189 (16): 6011–20. https://doi.org/10.1128/jb.00014-07.</ref>.
  
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===References===
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===Functional Parameters===
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<partinfo>BBa_K091001 parameters</partinfo>
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Latest revision as of 17:44, 26 July 2021

LsrR gene

This gene codes for the LsrR protein from E. coli strain MG1655. The protein is an repressor of the pLsrA promoter that can be derepressed upon binding to phosphorylated AI-2 signal molecule.

Information contributed by City of London UK (2021)

Part information is collated here to help future users of the BioBrick registry.

Metadata:

  • Group: City of London UK 2021
  • Author: Lucas Ng
  • Summary: Added information collated from existing scientific studies

The AI-2 signal quorum-sensing signal molecule is actively transported into the cell by the LsrABC protein, before being phosphorylated by LsrK. Subsequently, Phospho-AI-2 derepresses LsrR from the operator region of the lsr operon, as stated above. In the absence of AI-2, both the lsrACDBFG operon and LsrR itself are repressed [1].

References

  1. ↑ Li, Jun, Can Attila, Liang Wang, Thomas K. Wood, James J. Valdes, and William E. Bentley. 2007. “Quorum Sensing in Escherichia Coli Is Signaled by AI-2/LsrR: Effects on Small RNA and Biofilm Architecture.” Journal of Bacteriology 189 (16): 6011–20. https://doi.org/10.1128/jb.00014-07.