Difference between revisions of "Part:BBa F1610"

Latest revision as of 10:09, 20 October 2009

3OC₆HSL Sender Device

This device accepts PoPS as input and produces the LuxI enzyme. This enzyme produces 3OC₆HSL.

Notice

With a VF2-VR PCR length compraison, I found that it is empty on this plasmid sent in iGEM2007 plates. --marion 07:12, 22 March 2008 (EDT)

While present on a gel after running PCR on both 2007 and 2008 plates, the DNA appears to only be ~600bp long which conflicts with the part description. --robere 27 Jun 2008
See gel image

The part we used is in iGEM2009 plate. After cutting the plasmid in EcoRI and Spel restriction sites, we separated the fragments by electrophoresis. There appeared two bands, one is about 3100bp, and the other is about 800bp.--Koi 20 Oct 2009 (Tokyo_Tech)

See gel image

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 655
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 8: / Line 8: @@
 '''
 --[[User:MaRui|marion]] 07:12, 22 March 2008 (EDT)
-'''The part seems to be present in the iGEM2008 distribution, verified with VF2-VR PCR.
-'''
+'''While present on a gel after running PCR on both 2007 and 2008 plates, the DNA appears to only be ~600bp long which conflicts with the part description.''' --[[robere]] 27 Jun 2008<br>
+[[:Image:F1610_PCR_gel.jpg|See gel image]]
+'''The part we used is in iGEM2009 plate. After cutting the plasmid in EcoRI and Spel restriction sites, we separated the fragments by electrophoresis. There appeared two bands, one is about 3100bp, and the other is about 800bp.'''--[[User:Koi|Koi]]  20 Oct 2009 (Tokyo_Tech)
+[[:Image:F1610_restriction2009.jpg|See gel image]]
 <!-- Add more about the biology of this part here