Difference between revisions of "Part:BBa K2680116"
 
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<partinfo>BBa_K2680116 short</partinfo>
 
<partinfo>BBa_K2680116 short</partinfo>
  
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3G-compatible pLac/ara-1 dual input promoter. Repressed by LacI and activated by arabinose/AraC. Intended for use in oscillator creation. Sequence originally from Lutz and Bujard 1997; later used for the creation of oscillator from Stricker et al 2008. Note that the upstream LacO site is required for functional repression via LacI (tetrameter) [See Stricker et al 2008 supplement].
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pLac/ara-1 dual input promoter in a 3G-compatible form, flanked by sticky end A and suffix sticky end B in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].  
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Repressed by LacI and activated by arabinose/AraC. Intended for use in oscillator creation. Sequence originally from Lutz and Bujard 1997; later used for the creation of oscillator from Stricker et al 2008.<sup>1,2</sup> Note that the upstream LacO site is required for functional repression via LacI (tetrameter) [See Stricker et al 2008 supplement].
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===References===
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<sup>1</sup>Lutz, R., & Bujard, H. (1997). Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Research, 25(6), 1203–1210.
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<sup>2</sup>Stricker, J., Cookson, S., Bennett, M. R., Mather, W. H., Tsimring, L. S., & Hasty, J. (2008). A fast, robust and tunable synthetic gene oscillator. Nature, 456(7221), 516–519. https://doi.org/10.1038/nature07389
  
 
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Latest revision as of 21:29, 14 October 2018


3G pLac/ara-1

pLac/ara-1 dual input promoter in a 3G-compatible form, flanked by sticky end A and suffix sticky end B in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].

Repressed by LacI and activated by arabinose/AraC. Intended for use in oscillator creation. Sequence originally from Lutz and Bujard 1997; later used for the creation of oscillator from Stricker et al 2008.1,2 Note that the upstream LacO site is required for functional repression via LacI (tetrameter) [See Stricker et al 2008 supplement].

References

1Lutz, R., & Bujard, H. (1997). Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Research, 25(6), 1203–1210.

2Stricker, J., Cookson, S., Bennett, M. R., Mather, W. H., Tsimring, L. S., & Hasty, J. (2008). A fast, robust and tunable synthetic gene oscillator. Nature, 456(7221), 516–519. https://doi.org/10.1038/nature07389

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 476
    Illegal XhoI site found at 443
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 41
    Illegal BsaI.rc site found at 556