Difference between revisions of "Part:BBa K2559011"
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<partinfo>BBa_K2559011 short</partinfo>
 
<partinfo>BBa_K2559011 short</partinfo>
  
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It is a weak promoter found in E.coli, and we uesd it to express our cording gene regulatedly， the expression of target genes is low and avoid the risk of losing transformed algae strains for toxic effect caused by target genes.
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The BBa_K2559004 , PcaiF promoter is a weak endogenous promoter in Escherichia coli K 12.  
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===Usage and Biology===
 
===Usage and Biology===
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We obtained this promoter from a database named PromEC ( http://margalit.huji.ac.il/promec/index.html) .PromEC is an updated compilation of E. coli mRNA promoter sequences. It includes detailed information of genesites which have been experimentally identified mRNA transcriptional starting position in the E. coli chromosome, as well as the actual sequences of the promoters.
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We have built up a model to measure the expression intensity of these promoters. Eventually, we selected three promoters including: PrplJ, Pdapa and PcaiF based on the results of modeling PrplJ, Pdapa and PcaiF.We further made chimeric fluorescent fusion proteins between the promoter and GFP respectively and tested the fluorescent intensity driven by different promoters. The combination of PromEC and our modeling can be used as a efficient tool to figure out the information about interested promoters. The GFP expression in E.coli (DH5 α) and was driven by PrplJ, Pdapa and PcaiF promoter.
  
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[[File:Scau-china-2018-11.png|800px|thumb|center|Figure 1 The result of the fluorescent intensity measurement ]]
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[[File:Scau-china-2018-12.png|800px|thumb|center|Figure 2 Fluorescent intensity of eGFP driven by by rplj/dapa/caif promoter.]]
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The indensity of PcaiF is very weak and we hardly can observe the GFP fluorescent signal.
  
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The part is facilitate the in-depth research for other teams!
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2559011 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K2559004 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2559011 parameters</partinfo>
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<partinfo>BBa_K2559004 parameters</partinfo>
 
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Latest revision as of 18:13, 15 October 2018


PcaiF promoter

The BBa_K2559004 , PcaiF promoter is a weak endogenous promoter in Escherichia coli K 12.

Usage and Biology

We obtained this promoter from a database named PromEC ( http://margalit.huji.ac.il/promec/index.html) .PromEC is an updated compilation of E. coli mRNA promoter sequences. It includes detailed information of genesites which have been experimentally identified mRNA transcriptional starting position in the E. coli chromosome, as well as the actual sequences of the promoters. We have built up a model to measure the expression intensity of these promoters. Eventually, we selected three promoters including: PrplJ, Pdapa and PcaiF based on the results of modeling PrplJ, Pdapa and PcaiF.We further made chimeric fluorescent fusion proteins between the promoter and GFP respectively and tested the fluorescent intensity driven by different promoters. The combination of PromEC and our modeling can be used as a efficient tool to figure out the information about interested promoters. The GFP expression in E.coli (DH5 α) and was driven by PrplJ, Pdapa and PcaiF promoter.

Figure 1 The result of the fluorescent intensity measurement
Figure 2 Fluorescent intensity of eGFP driven by by rplj/dapa/caif promoter.

The indensity of PcaiF is very weak and we hardly can observe the GFP fluorescent signal.

The part is facilitate the in-depth research for other teams!


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]