Difference between revisions of "Part:BBa K2789003:Design"

(→‎References)
(→‎Source)
 
Line 15: Line 15:
 
All the parts come from igem registry resources.
 
All the parts come from igem registry resources.
 
Ccas: BBa_K608102
 
Ccas: BBa_K608102
 +
 
Ccar: BBa_K592002
 
Ccar: BBa_K592002
 +
 
Lac operator: BBa_C0010, BBa_C0012
 
Lac operator: BBa_C0010, BBa_C0012
 +
 
double terminator: BBa_B0015
 
double terminator: BBa_B0015
 +
 
non gate: BBa_Q04400
 
non gate: BBa_Q04400
 +
 
rbs:BBa_B0034
 
rbs:BBa_B0034
−
gfp: BBa_K082003
 
  
 +
gfp: BBa_K082003
  
 
===References===
 
===References===
 
We have condon-optimeized Ccas BBa_K2789009 for E.coli from BBa_K608102 and deleted the EcoRI and SpeI site in the gene sequence. We paste the website of BBa_K608102 (https://parts.igem.org/Part:BBa_K608102)
 
We have condon-optimeized Ccas BBa_K2789009 for E.coli from BBa_K608102 and deleted the EcoRI and SpeI site in the gene sequence. We paste the website of BBa_K608102 (https://parts.igem.org/Part:BBa_K608102)

Latest revision as of 05:44, 12 October 2018


lac operator+rbs+Ccas+terminator+Ccar+lac operator+rbs+terminator+cpcg+non gate+rbs+gfp+termiantor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1393
    Illegal BamHI site found at 2973
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 798
    Illegal AgeI site found at 1803
    Illegal AgeI site found at 1998
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5242


Design Notes

We have optimized the code in the Ccas and Ccar. And we deleted the EcoRI restriction site in the Ccas for future DNA ligation and digestion.


Source

All the parts come from igem registry resources. Ccas: BBa_K608102

Ccar: BBa_K592002

Lac operator: BBa_C0010, BBa_C0012

double terminator: BBa_B0015

non gate: BBa_Q04400

rbs:BBa_B0034

gfp: BBa_K082003

References

We have condon-optimeized Ccas BBa_K2789009 for E.coli from BBa_K608102 and deleted the EcoRI and SpeI site in the gene sequence. We paste the website of BBa_K608102 (https://parts.igem.org/Part:BBa_K608102)