Difference between revisions of "Part:BBa K2789001:Design"

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===Design Notes===
 
===Design Notes===
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We have optimized the code in the Ccas and Ccar. And we deleted the EcoRI restriction site in the Ccas for future DNA ligation and digestion.
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This year we have improved the part BBa_K608102 designed by the 11-Freiburg iGEM team. This is a light control component Ccas. We want to use this component, but there are illegal EcoRI and SpeI sites in the sequence that do not comply with the RFC10 standard. Since this component needs to be used with another part Ccar, there will be problems during the assembly process. This team also encountered this problem, and this part has not been characterized.
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===References===
 
===References===
  
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We have condon-optimeized Ccas(Green light receptor) for E.coli from BBa_K608102 and delete the EcoRI and SpeI site in the gene sequence.
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We have condon-optimeized Ccas BBa_K2789009 for E.coli from BBa_K608102 and deleted the EcoRI and SpeI site in the gene sequence. We paste the website of BBa_K608102 (https://parts.igem.org/Part:BBa_K608102:Design)
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And this part BBa_K2789001 is consists of lac operator , Ccas and Ccar (The downstream signal transducers to control gene expression)

Latest revision as of 14:09, 17 October 2018


lac operator+rbs+Ccas+terminator+Ccar+lac operator+rbs+terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1393
    Illegal BamHI site found at 2973
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 798
    Illegal AgeI site found at 1803
    Illegal AgeI site found at 1998
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This year we have improved the part BBa_K608102 designed by the 11-Freiburg iGEM team. This is a light control component Ccas. We want to use this component, but there are illegal EcoRI and SpeI sites in the sequence that do not comply with the RFC10 standard. Since this component needs to be used with another part Ccar, there will be problems during the assembly process. This team also encountered this problem, and this part has not been characterized.


Source

All the parts come from igem registry resources.

Ccas: BBa_K608102

Ccar: BBa_K592002

Lac operator: BBa_C0010, BBa_C0012

double terminator: BBa_B0015

rbs: BBa_B0034

References

We have condon-optimeized Ccas BBa_K2789009 for E.coli from BBa_K608102 and deleted the EcoRI and SpeI site in the gene sequence. We paste the website of BBa_K608102 (https://parts.igem.org/Part:BBa_K608102:Design)

And this part BBa_K2789001 is consists of lac operator , Ccas and Ccar (The downstream signal transducers to control gene expression)