Difference between revisions of "Part:BBa K2685006:Design"
 
(21 intermediate revisions by 2 users not shown)
Line 2: Line 2:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2685006 short</partinfo>
 
<partinfo>BBa_K2685006 short</partinfo>
−
    This parts can provide tetracycline tolerance to our constructive detecting chasis which including tetA and tetO genes.
+
−
    TetA encodes the tetracycline efflux pump protein, which selectively transports tetracycline from the cytoplasm to the periplasm in exchange for protons, thus limiting tetracycline's access to ribosomes within cells.They are proton power dependent, single polypeptide, drug-specific efflux pump, and it belong to the main promoting factor superfamily.
+
<p>This parts can provide tetracycline tolerance to our constructive detecting chasis which including tetA and tetO genes.</p>
−
    We also design some single sites mutation comparing with the original sequence that we have got from the biobrick bank.
+
<p>  TetA encodes the tetracycline efflux pump protein, which selectively transports tetracycline from thecytoplasm to the periplasm in </p><p>exchange for protons, thus limiting tetracycline's access to ribosomes within cells.They are proton power dependent, single polypeptide, drug-specific efflux pump, and it belong to the main promoting factor superfamily.</p>
−
 
+
<p>  We also design some single sites mutation comparing with the original sequence that we have got from the biobrick bank.</p>
−
<partinfo>BBa_K2685006 SequenceAndFeatures</partinfo>
+
−
 
+
  
 +
                    <div class="at-user__avatar" align="center">
 +
"https://static.igem.org/mediawiki/parts/e/e6/T--TUST_China--part_document_072.png"
 +
                    </div>
 
===Design Notes===
 
===Design Notes===
−
detecting tetracycline and pump out the tetracycline
+
Detecting tetracycline and pump out the tetracycline.It is the most important parts in the detection chasis.
−
 
+
−
 
+
  
 
===Source===
 
===Source===
  
−
from the kit plate
+
TetA is PCR from the biobrick which included in the kit plate.
  
 
===References===
 
===References===

Latest revision as of 19:52, 17 October 2018


TetO+TetA

This parts can provide tetracycline tolerance to our constructive detecting chasis which including tetA and tetO genes.

TetA encodes the tetracycline efflux pump protein, which selectively transports tetracycline from thecytoplasm to the periplasm in

exchange for protons, thus limiting tetracycline's access to ribosomes within cells.They are proton power dependent, single polypeptide, drug-specific efflux pump, and it belong to the main promoting factor superfamily.

We also design some single sites mutation comparing with the original sequence that we have got from the biobrick bank.

"T--TUST_China--part_document_072.png"

Design Notes

Detecting tetracycline and pump out the tetracycline.It is the most important parts in the detection chasis.

Source

TetA is PCR from the biobrick which included in the kit plate.

References