Difference between revisions of "Part:BBa K2683030"
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<partinfo>BBa_K2683030 short</partinfo> | <partinfo>BBa_K2683030 short</partinfo> | ||
| − | PPiase in | + | Peptidyl-prolyl isomerase (PPiase) is a protein that aids the isomerization of prolines in order to catalyze the addition proline amino acids onto the peptide chains from the aminoacyl-tRNA it is attached to [1]. This part has been codon optimized for use in <i> E.coli </i>, Contains a N-terminal his tag, and has a T7 promoter, medium strength RBS, and double terminator. This part was originally designed by the 2017 LEthbridge iGEm team (http://2017.igem.org/Team:Lethbridge). |
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| + | This part was improved by placing it into the pSB1C3 plasmid. | ||
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| + | https://static.igem.org/mediawiki/2018/6/6d/T--Lethbridge--PPiaseOE.PNG | ||
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| + | Figure 1- An overexpression gel of PPiase protein run on a 12% SDS-PAGE. Samples were taken at point of induction and then in 1 hour increments thereafter. The induced proteins is expected to be seen at about 22kDa range | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 04:00, 17 October 2018
Peptidyl Prolyl Isomerase (PPiase) in pSB1C3
Peptidyl-prolyl isomerase (PPiase) is a protein that aids the isomerization of prolines in order to catalyze the addition proline amino acids onto the peptide chains from the aminoacyl-tRNA it is attached to [1]. This part has been codon optimized for use in E.coli , Contains a N-terminal his tag, and has a T7 promoter, medium strength RBS, and double terminator. This part was originally designed by the 2017 LEthbridge iGEm team (http://2017.igem.org/Team:Lethbridge).
This part was improved by placing it into the pSB1C3 plasmid.
Figure 1- An overexpression gel of PPiase protein run on a 12% SDS-PAGE. Samples were taken at point of induction and then in 1 hour increments thereafter. The induced proteins is expected to be seen at about 22kDa range
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]