Difference between revisions of "Part:BBa K2683029"

Latest revision as of 02:01, 12 October 2018

Nucleoside Diphosphate Kinase (NDK) in pSB1C3

Nucleoside diphosphae kinase (NDK) is responsible for the catalysis of the exchange of a phosphate group between nucleoside diphosphates (NDP) and nucleoside triphosphates (NTP) [1]. This part is codon optimized for use in E.coli , has a N-terminal his tag and has a T7 promoter, medium strength RBS and double terminator. This part was originally designed by the 2017 Lethbridge team (http://2017.igem.org/Team:Lethbridge).

This part was improved by placing it into the pSB1C3 plasmid.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2683029 short</partinfo>
-NDK now in pSb1C3 standard
+Nucleoside diphosphae kinase (NDK) is responsible for the catalysis of the exchange of a phosphate group between nucleoside diphosphates (NDP) and nucleoside triphosphates (NTP) [1]. This part is codon optimized for use in <i> E.coli </i>, has a N-terminal his tag and has a T7 promoter, medium strength RBS and double terminator. This part was originally designed by the 2017 Lethbridge team (http://2017.igem.org/Team:Lethbridge).
+This part was improved by placing it into the pSB1C3 plasmid.
 <!-- Add more about the biology of this part here