Difference between revisions of "Part:BBa K2683026"

Latest revision as of 03:41, 17 October 2018

Elongation Factor- Thermo Unstable (EF-Tu) in pSB1C3

EF-Tu (elongation Factor thermo unstable) is a prokaryotic protein that aids the binding of an aminoacyl-tRNA (aa-tRNA) to the ribosome. Thus EF-Tu aids the elongation of peptide chains during protein synthesis [1]. This part is codon optimized for use in Escherichia coli , contains an N-terminal hexahistidine tag for purification and has a T7 Promoter, medium, strength RBS and double terminator. This part was originally designed by the 2017 Lethbridge iGEM team (http://2017.igem.org/Team:Lethbridge) and was initially characterized in the pUC57 plasmid (https://parts.igem.org/Part:BBa_K2443027).

This part was improved by placing the part in the pSB1C3 plasmid.

Figure 1- An overexpression gel of EF-Tu run on a 12% SDS-PAGE. Samples were taken at point of induction and in 1 hour increments thereafter. Although induction is weak, the protein is seen at the 45kDa range.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2683026 short</partinfo>
-EF-Tu now in the pSb1C3 standard
+EF-Tu (elongation Factor thermo unstable) is a prokaryotic protein that aids the binding of an aminoacyl-tRNA (aa-tRNA) to the ribosome. Thus EF-Tu aids the elongation of peptide chains during protein synthesis [1]. This part is codon optimized for use in <i>Escherichia coli </i>, contains an N-terminal hexahistidine tag for purification and has a  T7 Promoter, medium, strength RBS and double terminator. This part was originally designed by the 2017 Lethbridge iGEM team (http://2017.igem.org/Team:Lethbridge) and was initially characterized in the pUC57 plasmid (https://parts.igem.org/Part:BBa_K2443027).
+This part was improved by placing the part in the pSB1C3 plasmid.
+https://static.igem.org/mediawiki/2018/a/a0/T--Lethbridge--trpRSaOE.PNG
+Figure 1- An overexpression gel of EF-Tu run on a 12% SDS-PAGE. Samples were taken at point of induction and in 1 hour increments thereafter. Although induction is weak, the protein is seen at the 45kDa range.
 <!-- Add more about the biology of this part here