Difference between revisions of "Part:BBa K2683023"
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<partinfo>BBa_K2683023 short</partinfo> | <partinfo>BBa_K2683023 short</partinfo> | ||
− | + | Valine tRNA synthetase is responsible for attaching the valine amino acid onto its tRNA to form an aminoacyl-tRNA [1]. The part has been codon optimized for use in <i> Escherichia coli </i>, contains a C-terminal hexahistidine tag with a serine glycine linker and has a T7 Promoter, medium strength RBS and double terminator. These original designs were done by the 2017 Lethbridge iGEM team (http://2017.igem.org/Team:Lethbridge). | |
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+ | This part has been improved by inserting it into the pSB1C3 plasmid. | ||
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Latest revision as of 00:42, 12 October 2018
Valine tRNA Synthetase (ValRS) in pSB1C3
Valine tRNA synthetase is responsible for attaching the valine amino acid onto its tRNA to form an aminoacyl-tRNA [1]. The part has been codon optimized for use in Escherichia coli , contains a C-terminal hexahistidine tag with a serine glycine linker and has a T7 Promoter, medium strength RBS and double terminator. These original designs were done by the 2017 Lethbridge iGEM team (http://2017.igem.org/Team:Lethbridge).
This part has been improved by inserting it into the pSB1C3 plasmid.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2661