Difference between revisions of "Part:BBa K2692001:Design"

(→‎References)
(→‎Source)
 
(2 intermediate revisions by the same user not shown)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
The addition of a his tag for purification purposes
+
The coding sequence has been optimized for use in <i> E.coli </i>
−
 
+
−
 
+
  
 
===Source===
 
===Source===
−
 
+
https://www.uniprot.org/uniprot/P04535
−
The part was synthesized as a g-block (linear Piece of DNA) through IDT.
+
  
 
===References===
 
===References===
−
[1] Van der Vies, S., Gatenby, A and Georopoulos, C. (1994) Bacteriphage T4 encodes a co-chaperonin that can substitute for <i> Escherichia coli <i/> GroES in protein folding. Nature. 368, 654-656
+
[1]Olson N., Gingery, M., Eiserling, F., and Baker, T. (2001) The structure of isometric capsids of bacteriphage T4. Virology. 279,385-391

Latest revision as of 05:09, 16 October 2018


Major T4 Capsid Protein gp23


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 9
    Illegal PstI site found at 342
    Illegal PstI site found at 1524
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9
    Illegal PstI site found at 342
    Illegal PstI site found at 1524
    Illegal NotI site found at 15
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9
    Illegal BamHI site found at 1069
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 9
    Illegal PstI site found at 342
    Illegal PstI site found at 1524
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 9
    Illegal XbaI site found at 24
    Illegal PstI site found at 342
    Illegal PstI site found at 1524
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1118


Design Notes

The coding sequence has been optimized for use in E.coli

Source

https://www.uniprot.org/uniprot/P04535

References

[1]Olson N., Gingery, M., Eiserling, F., and Baker, T. (2001) The structure of isometric capsids of bacteriphage T4. Virology. 279,385-391