Difference between revisions of "Part:BBa K2633006:Experience"

(→‎Applications of BBa_K2633006)
 
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https://static.igem.org/mediawiki/parts/d/db/T--MichiganState--transformation_results_small.png
 
https://static.igem.org/mediawiki/parts/d/db/T--MichiganState--transformation_results_small.png
  
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Figure 2.[https://static.igem.org/mediawiki/parts/thumb/1/13/T--MichiganState--root_colonization_3wk_GA_results_full.png/900px-T--MichiganState--root_colonization_3wk_GA_results_full.png] 3 weeks post inoculation on plant roots. A) FCP2-01 forming large colonies abount 2 mm long in ''B. distachyon'' roots
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Figure 2.[https://static.igem.org/mediawiki/parts/thumb/1/13/T--MichiganState--root_colonization_3wk_GA_results_full.png/900px-T--MichiganState--root_colonization_3wk_GA_results_full.png] 3 weeks post inoculation on plant roots. A) FCP2-01 forming large colonies about 2 mm long in ''B. distachyon'' roots
 
B) Detail of panel A. C) FCP2-01 growing between plant cells on the root surface. D) Detail of colonies on root epidermis.
 
B) Detail of panel A. C) FCP2-01 growing between plant cells on the root surface. D) Detail of colonies on root epidermis.
 
   
 
   

Latest revision as of 01:49, 16 October 2018


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Applications of BBa_K2633006

This part was transformed using a restriction site illegal version of BBa_K2633000[1]. It was then used to inoculate Brachypodium distachyon seeds. Transformed bacteria expressing GFP were observed on and inside roots from 1 to 4 weeks post inoculation.

Figure 1. A) FCP2-01 transformed with GFP (left) vs wild-type (right) under blue light with orange filter. B) Transformed colonies on an agar plate.

T--MichiganState--transformation_results_small.png

Figure 2.[2] 3 weeks post inoculation on plant roots. A) FCP2-01 forming large colonies about 2 mm long in B. distachyon roots B) Detail of panel A. C) FCP2-01 growing between plant cells on the root surface. D) Detail of colonies on root epidermis.

T--MichiganState--root_colonization_3wk_GA_results_small.png

Figure 3.[3] 4 weeks post inoculation on plant roots. A) FCP2-01 on B. distachyon roots. B) FCP2-0 colonizing root hairs. C) FCP2-01 growing on epidermis of the root. D) Enlarged image of panel C.

T--MichiganState--root_colonization_4wk_GA_results_small.png

User Reviews

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